Molecular Biology & Gene Expression
We offer the following services for amplification, cloning, expression of genes and proteins:
Recombinant DNA services
- PCR (Polymerase Chain Reaction) based amplification, cloning and sequence verification
- Characterisation of gene expression by northern hybridisation, dot blots and slot blots
- Construction of custom vectors (for expression, etc.) through vector engineering
- Genotyping using PCR analysis (e.g. Mouse tail PCR)
- Site directed mutagenesis
- Custom cDNA library preparation
- An array of 40 odd human cell lines to quickly source RNA
Gene expression
- E. coli (Bl21, shake flask)
- Yeast (Saccharomyces)
- Insect cell (Bac to bac system)
- Mammalian hosts
Yeast biology
- Function determination
- Yeast models
- Customised screens with human genes
- Two hybrid screens
