 Human
whole blood assays
 Cycloxygenase 1 and 2
5-Lipoxygenase
Monocytic cytokines IL-1,TNFα, IL-12, IL-6
Lymphocytic cytokines IFNγ, IL-2, IL-10
Other cytokines interleukins and growth factors
In vitro enzymatic assay
Cycloxygenase 1 and 2
5-Lipoxygenase
Phospholipase A2
Chemokines and chemotaxis assays
IL-8
Eotaxin
MCP-1 mediated THP-1 chemotaxis
Cell adhesion
VLA4, VLA5
ICAM-1, VCAM-1
E-selectin,
P-selectin
Intracellular calcium
PAF induced intracellular calcium in human platelets
LTB4
induced intracellular calcium in U937 cells
Phosphodiesterases
In vitro SPA/ELISA based enzymatic assay: PDE 3,4,5,9
Kinases
p38MAPK
PKC
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Mitogen
induced T & B cell proliferation - [3H] thymidine
incorporation assay
PHA induced T cell & LPS induced B cell
lymphoproliferation
Proteases
TACE
MMP9
Cathepsin B
Cathepsin S
Human GPCRs binding and functional assay
Cannabinoid1 &2
Endothelin B
Histamine H1 & H3
Neurokinin 1
Muscarinic 2
Platelet activating factor |
